Journal: Cell Death & Disease

Article Title: Exosomal circRNA_100284 from arsenite-transformed cells, via microRNA-217 regulation of EZH2, is involved in the malignant transformation of human hepatic cells by accelerating the cell cycle and promoting cell proliferation

doi: 10.1038/s41419-018-0485-1

Figure Lengend Snippet: CM-exo exosomes derived from passage-control L-02 cells, T-CM-exo exosomes derived from arsenite-transformed L-02 cells. Exosomes from C-L-02 cells and T-L-02 cells were fractionated by Exoquick. a Representative electron micrographs of CM-exo or T-CM-exo (right, bars = 100 nm). b Particle number and size analysis of CM-exo or T-CM-exo were determined by dynamic light scattering using a ZetaView® nanoparticle tracker (ParticleMetrix GmbH; Meerbusch, Germany). c Western blots of CD9 and CD81 in cells, CM-exo or T-CM-exo. d circRNA_100284 levels in CM-exo or T-CM-exo were measured by qRT-PCR (means ± SD, n = 3). * P < 0.05, different from CM-exo. CM-exo and T-CM-exo were labeled with PKH67, a green fluorescent cell linker. e Normal L-02 cells after 3 h incubation of exosomes with fluorescently labeled PKH67. Green represents CM-exo or T-CM-exo staining by PKH67, and blue represents nuclear DNA staining by DAPI

Article Snippet: Exosomes from C-L-02 cells and T-L-02 cells were fractionated by Exoquick. a Representative electron micrographs of CM-exo or T-CM-exo (right, bars = 100 nm). b Particle number and size analysis of CM-exo or T-CM-exo were determined by dynamic light scattering using a ZetaView® nanoparticle tracker (ParticleMetrix GmbH; Meerbusch, Germany). c Western blots of CD9 and CD81 in cells, CM-exo or T-CM-exo. d circRNA_100284 levels in CM-exo or T-CM-exo were measured by qRT-PCR (means ± SD, n = 3).

Techniques: Derivative Assay, Control, Transformation Assay, Western Blot, Quantitative RT-PCR, Labeling, Incubation, Staining

Journal: Journal of Extracellular Vesicles

Article Title: Optimized culture methods for isolating small extracellular vesicles derived from human induced pluripotent stem cells

doi: 10.1002/jev2.12065

Figure Lengend Snippet: Identification of the characteristics of sEVs. (a‐c) Transmission electron microscopy (TEM), nanoparticle tracking analysis (NTA) and western blotting were used to examine the characteristics of sEVs continuously collected for 5 days. (d‐f) TEM, dynamic light scattering (DLS) and CytoFLEX flow cytometer were used to examine the characteristics of sEVs extracted on day 1, day 3, and day 5. (a) TEM results of sEVs. M‐UC‐ex represents sEVs extracted by M‐UC; EQ‐ex represents the sEVs extracted by EQ. Bar = 200 nm. (b) NTA results of sEVs derived from hiPSCs‐EC1. (c) The western blotting results. HSP70, CD63, and TSG101 are the exosome marker proteins; Calreticulin is the negative control. (d) TEM results of sEVs. Bar = 200 nm. (e) DLS results of sEVs. (f) CytoFLEX flow cytometer results of sEVs derived from hiPSCs‐EC1

Article Snippet: The average particle dimensions and concentration of the sEVs were characterized by the DLS technique (ZetaView, Particle Metrix).

Techniques: Transmission Assay, Electron Microscopy, Western Blot, Flow Cytometry, Derivative Assay, Marker, Negative Control